Detection of Hybrids between Bull Trout (Salvelinus confluentus) and Brook Trout (Salvelinus fontinalis) Using PCR Primers Complementary to Interspersed Nuclear Elements

Paul Spruell; Meredith L. Bartron; Naohisa Kanda; Fred W. Allendorf

doi:10.1643/0045-8511(2001)001[1093:DOHBBT]2.0.CO;2

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Paul Spruell,¹ Meredith L. Bartron,¹ Naohisa Kanda,¹ Fred W. Allendorf¹

¹Wild Trout and Salmon Genetics Laboratory, Division of Biological Sciences, University of Montana, Missoula, Montana 59812. Present addresses: (MLB) Michigan State University, Department of Fisheries and Wildlife, 13 Natural Resources Building, East

Abstract

We used four pairs of polymerase chain reaction primers complementary to the ends of interspersed nuclear elements to amplify markers that differentiate bull trout (Salvelinus confluentus) and brook trout (S. fontinalis) and allow the identification of hybrids between these species. We identified 21 DNA fragments in bull trout that are absent from brook trout and 13 DNA fragments in brook trout that are absent from bull trout. This PCR-based test gave results that are consistent with results from allozyme loci known to be diagnostic for these species. Similar interspersed elements are found in all eukaryotes, making this a potentially useful technique for species identification in many taxa.

The American Society of Ichthyologists and Herpetologists

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Paul Spruell, Meredith L. Bartron, Naohisa Kanda, and Fred W. Allendorf "Detection of Hybrids between Bull Trout (Salvelinus confluentus) and Brook Trout (Salvelinus fontinalis) Using PCR Primers Complementary to Interspersed Nuclear Elements," Copeia 2001(4), 1093-1099, (1 December 2001). https://doi.org/10.1643/0045-8511(2001)001[1093:DOHBBT]2.0.CO;2

Accepted: 10 April 2001; Published: 1 December 2001

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